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SYBR® Green Extract-N-Amp™ Plant PCR Kit
sufficient for 100 preparations
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About This Item
Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55
Service technique
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Laissez-nous vous aiderUtilisation
sufficient for 100 preparations
sufficient for 100 reactions
Niveau de qualité
Caractéristiques
dNTPs included
hotstart
Technique(s)
qPCR: suitable
Méthode de détection
SYBR® Green
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
SYBR® Green Extract-N-Amp™ Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
Caractéristiques et avantages
- Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- No loading buffers or tracking dyes required for gel analysis
- SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Compatible with high-throughput requirements for genetic analysis of plants
- No long enzymatic digestions
- Extract stable at 4°C for at least 6 months
Autres remarques
The protocol uses a 20 μl PCR reaction.
Informations légales
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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