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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
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Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1E20, recombinant monoclonal

Description

1E20 Clone

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 90.07 kDa
observed mol wt ~80 kDa

Produit purifié par

using Protein A

Espèces réactives

human, monkey

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Technique(s)

affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

C-terminal

Numéro d'accès Protein ID

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Informations sur le gène

human ... RRM1(6240)

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1E20 is ZooMAb® rabbit recombinant monoclonal antibody that specifically detects RRM1. It targets an epitope within 22 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Ribonucleotide reductase large subunit (RRM1).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RRM1 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RRM1 in A549 and COS-7 cell lysates.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected RRM1 in one million HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RRM1 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound RRM1 with an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Ribonucleoside-diphosphate reductase large subunit (UniProt: P23921; also known as EC:1.17.4.1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit, RRM1) is encoded by the RRM1 (also known as RR1) gene (Gene ID: 6240) in human. RRM1 is a member of the ribonucleoside diphosphate reductase large chain family. Ribonucleotide reductase (RNR) catalyzes the rate-limiting step of de novo nucleotide synthesis through reduction of ADP, CDP, GDP, and UDP to their deoxynucleotide equivalents. The RNR complex consists of catalytic RRM1 dimers in complex with a smaller subunit RRM2.  Two distinct regulatory sites have been defined for RRM1: the specificity site that controls substrate specificity;  and the activity site that regulates its overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. RRM1 is functionally associated with DNA replication and DNA damage repair. Level of its enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle.  Higher expression of RRM1 has been linked with poor clinical outcome and poor overall survival on adjuvant chemotherapy in patients with pancreatic cancer.  Cancer cells lacking cytoplasmic RRM1 activation exhibit severe DNA damage. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shintaku, J., et al. (2022). J. Clin. Invest. 132(13):e145660; Kato, T., et al. (2021). PLoS ONE 16(6); e0252917; Bepler, G., et al. (2006). J. Clin. Oncol. 24(29); 4731-4737).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Certificats d'analyse (COA)

Lot/Batch Number

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