ZRB2901

Anti-LDLRAD3 Antibody, clone 2N16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : LDLR class A domain-containing protein 3, Low-density lipoprotein receptor class A domain-containing protein 3
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2N16, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 37.41 kDa; observed mol wt ~37 kDa
• Produit purifié par: using Protein A
• Espèces réactives: rat, human
• Réactivité de l'espèce (prédite par homologie): bovine
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal cytoplasmic domain
• Numéro d'accès Protein ID: NP_777562.1
• Numéro d'accès UniProt: Q86YD5
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... LDLRAD3(143458)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 2N16 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects LDLRAD3. It targets an epitope within 22 amino acids from the C-terminal cytoplasmic domain.

Immunogène

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal cytoplasmic domain of human LDLRAD3.

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LDLRAD3 in A549 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LDLRAD3 in lysates from SK-MEL-28 cells, human lung tissue, human stomach tissue, and rat lung tissue.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected LDLRAD3 in human fallopian tube tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LDLRAD3 in human fallopian tube tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound LDLRAD3 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Low-density lipoprotein receptor class A domain-containing protein 3 (UniProt: Q86YD5; also known as LDLR class A domain-containing protein 3) is encoded by the LDLRAD3 (also known as LRAD3 ) gene (Gene ID: 143458) in human. LDLRAD3 is a protein-coding gene and is located on the 11p13 chromosome. It is known to be associated with amyloid-beta binding. The encoded protein is a member of the LDL receptor family and is involved in lipid metabolism, cellular signaling, and may also interact with other receptors and proteins, influencing various signaling cascades. It is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 18-173), a transmembrane domain (aa 174-194), and a cytoplasmic tail (aa 195-345). LDLRAD3 is expressed in various tissues, with higher levels noted in brain, lung, skeletal muscle, and pancreas. It is also found in moderate levels in heart, placenta, and kidney but not detected in the liver. LDLRAD3 is involved in several processes, may regulate ITCH and NEDD4 E3 ligase activity and degradation. Studies have established LDLRAD3 as a potent activator of ITCH and NEDD4, as evidenced by their increased auto-ubiquitination when bound to LRAD3. It has also been suggested that circular RNA circ-LDLRAD3 may also be a potential biomarker in the diagnosis of pancreatic cancer. LDLRAD3 is mainly associated with microbial infection and acts as a entry receptor for Venezuelan equine encephalitis virus (VEEV), which is a New World alphavirus that causes severe neurological disease in humans. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Basore, K., et al. (2021). Nature. 598:672-676; Noyes, NC., et al. (2016). Biochemistry. 55(8):1204-13; Yang, F., et al. (2017). World J Gastroenterology. 23(47):8345-8354).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2