50631-U

Ascentis^® Express 90 Å F5 (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonyme(s) : Core-shell (SPP) Fused Core PFP HPLC column
• Code UNSPSC : 41115700
• eCl@ss : 32110501
• Nomenclature NACRES : SB.52

Propriétés

• Product Name: Ascentis^® Express F5, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• Matériaux: stainless steel column
• Niveau de qualité: 100
• Agence: suitable for USP L43
• Gamme de produits: Ascentis^®
• Caractéristiques: endcapped
• Fabricant/nom de marque: Ascentis^®
• Conditionnement: 1 ea of
• Paramètres: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• Technique(s): HPLC: suitable; LC/MS: suitable
• L × D.I.: 15 cm × 4.6 mm
• Superficie: 90 m²/g
• Impuretés: <5 ppm metals
• Matrice: Fused-Core particle platform; superficially porous particle
• Groupe de la matrice active: PFP (pentafluorophenyl) phase
• Taille des particules: 5 μm
• Dimension de pores: 90 Å pore size
• pH de fonctionnement: 2-8
• Application(s): food and beverages
• Technique de séparation: reversed phase

Description

Description générale

Ascentis^® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

• Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employed the Ascentis^® Express F5, 5 µm HPLC column to determine lumacaftor and its degradation products in the presence of ivacaftor. The application of high-performance liquid chromatography ion trap time-of-flight mass spectrometry provided high sensitivity and resolution, showcasing the column′s efficacy in complex sample matrices (Özcan et al., 2023).


• Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This research highlights the use of the Ascentis^® Express F5, 5 µm HPLC column in developing stability-indicating methods for tasimelteon. The column′s performance in both LC-MS/MS and LC-DAD methods ensured accurate and reliable identification of degradation products (Özcan et al., 2020).


• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in HPLC technology, including the Ascentis^® Express F5, 5 µm HPLC column. The article emphasizes the column′s capability in ultra-fast separations, providing enhanced efficiency and resolution in various analytical applications (Ali et al., 2012).

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Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H314
• Conseils de prudence: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Classification des risques: Eye Dam. 1 - Skin Corr. 1B
• Code de la classe de stockage: 8B - Non-combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable