I1646

IL-3 human

≥97% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): Interleukin-3 human, hIL-3, IL-3
• MDL number: MFCD00147276
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Interleukin-3 human, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (HPLC and SDS-PAGE)
• form: lyophilized powder
• potency: ≤1.0 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: 15 kDa
• packaging: pkg of 10 and 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.1 EU/μg
• UniProt accession no.: P08700
• storage temp.: −20°C
• Gene Information: human ... IL3(3562)

Description

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Interleukin-3 is a multifunctional protein produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and induce IgG secretion from activated B cells.

Physical form

Lyophilized with no additives. Sterile filtered through a 0.2 μm filter.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves