SRP3275

IL-33 human

Animal-component free, ≥98% (SDS-PAGE & HPLC), recombinant, expressed in E. coli, lyophilized

• Synonym(s): NF-HEV
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Product Name: IL-33 human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: ≥0.05 ng/mL ED₅₀
• mol wt: 17.9 kDa
• packaging: pkg of 10 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: O95760
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL33(90865)

Description

General description

It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.

Biochem/physiol Actions

Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. Recombinant human IL-33 is a 17.9 kDa protein containing 159 amino acid residues.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 8.0 + 0.5 mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable