MAB2058

Anti-Integrin β4 Antibody, clone ASC-3

clone ASC-3, Chemicon^®, from mouse

• Sinónimos: CD104
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: ASC-3, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Chemicon^®
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NM_000213.3; NM_001005619.1; NM_001005731.1
• Nº de acceso UniProt: P16144
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ITGB4(3691)

Descripción

Especificidad

Human beta4 integrin (CD104)

Inmunógeno

Human squamous cell carcinoma cells (SCC9)

Aplicación

Detect Integrin β4 using this Anti-Integrin β4 Antibody, clone ASC-3 validated for use in FC, IP, IH.

Inhibition of cell attachment: inhibits SCC9 cell adhesion to human

laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.

Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.

Working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Ligadura / enlace

Replaces: 05-283

Forma física

Format: Purified

Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable