MAB2059

Anti-Integrin β4 Antibody, clone ASC-8

clone ASC-8, Chemicon^®, from mouse

• Sinónimos: CD104, MAB2059Z
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: ASC-8, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Chemicon^®
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NM_000213.3; NM_001005619.1; NM_001005731.1
• Nº de acceso UniProt: P16144
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ITGB4(3691)

Descripción

Especificidad

Human beta4 integrin (CD104)

Inmunógeno

Human squamous cell carcinoma cells (SCC9)

Aplicación

Immunoprecipitation: A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).

Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).

Working dilutions must be determined by end user.

Anti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.

Research Category
Cell Structure

Research Sub Category
Integrins

Calidad

Flow Cytometry Analysis:
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.

Ligadura / enlace

Replaces: 05-283

Forma física

Format: Purified

Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable