324713

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

• Synonym(e): Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus, V8 Protease
• CAS Number: 66676-43-5
• EG-Nummer: 3.4.21.19 (BRENDA, IUBMB)
• MDL-Nummer: MFCD01324998
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Staphylococcus aureus
• Qualitätsniveau: 100
• Qualität: Proteomics Grade
• Form: lyophilized
• Spezifische Aktivität: ≥10 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α₂-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Warnhinweis

Toxicity: Harmful (C)

Einheitendefinition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Sonstige Hinweise

Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA69, 3506.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H317,H334
• P-Sätze: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Gefahreneinstufungen: Resp. Sens. 1 - Skin Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable