T6400

Tris-Borat-EDTA-Puffer

5× Concentrate

• Synonym(e): TBE buffer
• MDL-Nummer: MFCD00236358
• UNSPSC-Code: 41105319
• PubChem Substanz-ID: 329826638
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 200
• Sterilität: 0.2 μm filtered
• Form: solution
• Verunreinigungen: DNase and RNase, none detected
• pH-Wert: 8.2-8.4 (25 °C)
• SMILES String: OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
• InChIKey: OSBLTNPMIGYQGY-UHFFFAOYSA-N

Beschreibung

Anwendung

Ready for use in gel electrophoresis after dilution to working concentrations.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Tris-Borate-EDTA buffer has been used in the electrophoresis of the plasmid extracted from activated sludge.

Verpackung

Supplied in dispenser with a spigot.

Sonstige Hinweise

0.445 M Tris borate, pH approx. 8.3, containing 0.01 M EDTA.

Angaben zur Herstellung

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Solution prepared with 18 megohm water