SRP0734

PIP4K2B, Active

PRECISIO^® Kinase, recombinant

• Código UNSPSC: 12352202
• NACRES: NA.27

Propiedades

• recombinante: expressed in baculovirus infected Sf9 cells
• Ensayo: 70-95% (SDS-PAGE)
• Formulario: frozen liquid
• actividad específica: 4500-6000 nmol/min·mg (ADP-Glo)
• mol peso: ~73 kDa
• concentración: 0.1 μg/μL
• nº de acceso: NM_003559
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PIP4K2B(8396)

Descripción

Aplicación

Useful for kinase assay.

Acciones bioquímicas o fisiológicas

Recombinant full-length human PIP4K2B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP4K2B (phosphatidylinositol-5-phosphate 4-kinase, type II, beta) catalyzes the phosphorylation of phospha-tidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. PIP4K2B is a member of the phosphatidylinositol-5-phosphate 4-kinase family. PIP5K2B interacts specifically with the juxtamembrane region of the p55 TNF receptor and that PIP5K2B activity is increased in mammalian cells by treatment with TNF-alpha ⁽¹⁾. PIP5P4KB plays a role in determining insulin sensitivity and adiposity ⁽²⁾.

Reconstitución

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Almacenamiento y estabilidad

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Información legal

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable