06-661

Anti-Calreticulin Antibody

Upstate^®, from rabbit

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: pig, human, mouse, Xenopus, bovine, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_004343
• UniProt accession no.: P27797
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CALR(811)

Description

Specificity

calreticulin; does not cross react with other endoplasmic reticulum proteins

Immunogen

peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin

Application

Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.

Research Sub Category
PI3K, Akt, & mTOR Signaling

Enzymes & Biochemistry

Quality

routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts

Target description

60kDa

Linkage

Replaces: MABT145

Physical form

0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.

Format: Purified

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable