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Merck

M8816

Sigma-Aldrich

Microsomes from Liver, Pooled

from Monkey(Cynomolgus spp.), male

Synonym(s):

lab equipment accessory

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About This Item

UNSPSC Code:
12161501
NACRES:
NB.35
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biological source

Monkey (Cynomolgus spp.)

form

liquid

specific activity

≥5000 units/mg protein

packaging

vial of ~10 mg

shipped in

dry ice

storage temp.

−70°C

Application

Microsomes from Liver, Pooled has been used to measure the in vitro stabilities of the prodrugs. It has also been used in microsomal incubation to generate BTX-3 metabolites to serve as references for liquid chromatography with tandem mass spectrometry (LC-MS/MS) analyses of urine.

Biochem/physiol Actions

Liver microsomes are subcellular particles derived from the endoplasmic reticulum of hepatic cells. These microsomes are a rich source of drug metabolizing enzymes, including cytochrome P-450. Microsome pools from various sources are useful in the study of xenobiotic metabolism and drug interactions.
Microsomal cytochrome P450 proteins can catalyze the transfer of a single oxygen atom to endogenous and exogenous compounds, like steroids and phenobarbital, meant for excretion and detoxification.
Source of drug metabolizing enzymes, including cytochrome P-450.

Unit Definition

One unit will produce 1.0 picomole of 6 beta-hydroxytestosterone per minute at pH 7.4 at 37 deg C.


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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable



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