341738

Sulforhodamine B, acid form

Dye content 95 %

• Empirical Formula (Hill Notation): C₂₇H₃₀N₂O₇S₂
• CAS Number: 2609-88-3
• Molecular Weight: 558.67
• EC Number: 220-025-2
• MDL number: MFCD00067690
• UNSPSC Code: 12352103
• PubChem Substance ID: 57649121
• NACRES: NA.23

Properties

• form: solid
• Quality Level: 200
• composition: Dye content, 95%
• mp: >300 °C (lit.)
• λmax: 558 nm
• SMILES string: CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1
• InChI: 1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)
• InChI key: IOOMXAQUNPWDLL-UHFFFAOYSA-N

Description

General description

Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself into the solution under mild acidic conditions.

Application

pH sensitive of SRB towards it reactivity to living cells renders it useful for scaling drug induced cytotoxicity and cell proliferations. SRB assay was used to monitor the enzymatic activity of living cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves