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Sigma-Aldrich

Rap1 Activation Assay Kit

Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Pricing and availability is not currently available.
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Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable
affinity binding assay: suitable

NCBI accession no.

NM_021159.3

UniProt accession no.

P52306

shipped in

dry ice

Gene Information

human ... RAP1GDS1(5910)

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Rap1 Activation Assay Kit Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.

17-321

Rap1 Activation Assay Kit

Ral Activation Assay Kit Non-radioactive Ral Activation Assay Kit can be used to precipitate Ral-A-GTP from cell lysates & detection by a Ral-A specific antibody.

17-300

Ral Activation Assay Kit

Cdc42 Activation Assay Kit Non-radioactive cdc42 Activation Assay Kit can be used to precipitate cdc42-GTP from cell lysates subjected to various treatments & detection by a cdc42 specific monoclonal antibody.

17-286

Cdc42 Activation Assay Kit

Rac1 Activation Assay Kit Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

17-283

Rac1 Activation Assay Kit

technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

activity assay: suitable, affinity binding assay: suitable

Gene Information

human ... RAP1GDS1(5910)

Gene Information

human ... RALA(5898)

Gene Information

human ... CDC42(998)

Gene Information

human ... RAC1(5879)

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

NCBI accession no.

NM_021159.3

NCBI accession no.

NM_005402.2

NCBI accession no.

NM_001039802.1, NM_001791.3, NM_044472.2

NCBI accession no.

NM_006908.3, NM_018890.2, NM_198829.1

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

Packaging

Kit capacity: 20-30 assays

Components

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Rap1 Activation Lysis Buffer, 2X

Anti-Rap1

Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)

Quality

Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

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    Maddrell ER, et al.
    Journal of Nuclear Materials, 517, 71-79 (2019)

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