203806

5-Bromo-2′-deoxyuridine

Thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine.

• Synonym(s): 5-Bromo-2′-deoxyuridine, 5-BrdU
• CAS Number: 59-14-3
• MDL number: MFCD00006529
• UNSPSC Code: 12352208
• NACRES: NA.51

Properties

• description: RTECS - YU7350000
• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 10 mg/mL; 0.1 M NaOH: 50 mg/mL; DMSO: 50 mg/mL
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: Brc1[c]([nH][c]([n](c1)C2OC(C(C2)O)CO)=O)=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)
• InChI key: WOVKYSAHUYNSMH-UHFFFAOYSA-N

Description

General description

Thymidine analog. Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Can be used both in vitro and in vivo to label DNA. The resulting DNA can be used in conjuction with BrdU (Ab-2) Monoclonal Antibody (Cat. No. NA20) or BrdU Immunohistochemical System (Cat. No. HCS24) for the immunohistochemical analysis of cell proliferation.

Thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Can be used both in vitro and in vivo to label DNA. The resulting DNA can be used in conjunction with BrdU (Ab-2) Monoclonal Antibody (Cat. No. NA20) for the immunohistochemical analysis of cell proliferation.

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Reconstitution

Following reconstitution, sterilize by filtration through a 0.22 µm filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Garabedian, E.M., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15382.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Fukuda, K., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H340,H361fd
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 1B - Repr. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2