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407711

Sigma-Aldrich

Interleukin-12, Human, Recombinant, S. frugiperda

Synonym(s):

rhIL-12

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
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Assay

≥95% (SDS-PAGE)

Quality Level

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1.0 EU/μg Endotoxin (EU/μg IL-12)

shipped in

ambient

storage temp.

−70°C

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M3529A2010A6376
form

powder

form

powder

form

powder

form

powder

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥99% (TLC)

technique(s)

ligand binding assay: suitable

technique(s)

ligand binding assay: suitable

technique(s)

ligand binding assay: suitable

technique(s)

ligand binding assay: suitable

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

color

white to off-white

color

white to faint yellow

color

colorless to white

color

white to off-white

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B cells, and connective tissue-type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T cell differentiation and function.

Biochem/physiol Actions

ED₅₀ = 50-200 pg/ml as measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-12.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Other Notes

Kang, K., et al. 1996. J. Immunol. 156, 1402.
Riemann, H., et al. 1996. J. Immunol. 156, 1799.
Brunda, M.J., et al. 1994. J. Leukoc. Biol. 55, 280.
Smith, T.J., et al. 1994. Eur. J. Immunol. 24, 822.
Gubler, U., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 4143.
Stern, A.S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 6808.
Kobayashi, M., et al. 1989. J. Exp. Med. 170, 827.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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The oral obligate anaerobe Porphyromonas gingivalis possesses a small conserved transcript PG_RS02100 of unknown function we previously identified using small RNA-seq analysis as expressed during logarithmic growth. In this study, we sought to determine if PG_RS02100 plays a role in

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