553525

RAD51 Inhibitor, B02

The RAD51 Inhibitor, B02 controls the biological activity of RAD51. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(s): RAD51 Inhibitor, B02, ( E)-3-Benzyl-2-(2-(pyridin-3-yl)vinyl)quinazolin-4(3H)-one
• Empirical Formula (Hill Notation): C₂₂H₁₇N₃O
• CAS Number: 1290541-46-6
• Molecular Weight: 339.39
• MDL number: MFCD03294274
• UNSPSC Code: 12352200
• NACRES: NA.28

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: light yellow
• solubility: DMSO: 50 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [n]2([c](c3c(nc2\C=C\c4cnccc4)cccc3)=O)Cc1ccccc1
• InChI: 1S/C22H17N3O/c26-22-19-10-4-5-11-20(19)24-21(13-12-17-9-6-14-23-15-17)25(22)16-18-7-2-1-3-8-18/h1-15H,16H2/b13-12+
• InChI key: GEKDQXSPTHHANP-OUKQBFOZSA-N

Description

General description

A cell-permeable pyridinylvinyl-quinazolinone compound that is shown to specifically inhibit human RAD51 (IC₅₀ = 27.4 µM). Does not affect RecA even at much higher concentration (~250 µM). Directly interacts with RAD51 (K_d = 5.6 µM), and disrupts its binding to DNA and nucleoprotein filament formation. Blocks double-strand break-induced homologous recombination and enhances sensitivity of cells to Cisplatin (Cat. No. 232120) and Mitomycin C (Cat. No. 47589), (Cat. No. 475820). Diminishes co-aggregate formation between RAD51-ssDNA filament and dsDNA. RAD51-Stimulatory Compound-1, RS-1 (Cat. No. 553510) is also available.

Packaging

Packaged under inert gas

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Huang, F., et al. 2012. J. Med. Chem.55, 3031.
Huang, F., et al. 2011. ACS Chem. Biol.6, 628.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable