71341

pRSFDuet-1 DNA - Novagen

• Synonym(s): pRSFDuet-1 Vector
• UNSPSC Code: 41106602
• NACRES: NA.51

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• Promoter: Promoter name: T7
• selection: kanamycin
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.

Application

pRSFDuet-1 DNA - Novagen has been used as a vector:

• to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression
• to clone the coding region of Mad1597–719
• to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable