ABE1062

Anti-monomethyl Histone H2A.Z Antibody (Lys 7)

from rabbit, purified by affinity chromatography

• Synonym(s): Histone H2A.Z, H2A/z
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_002097
• UniProt accession no.: P0C0S5

Description

General description

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human monomethyl Histone H2A.Z (Lys 7) near the N-terminal.

Application

Anti-monomethyl Histone H2A.Z (Lys 7) is an antibody against monomethyl Histone H2A.Z (Lys 7) for use in WB, ICC, DB, IP.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected monomethyl Histone H2A.Z (Lys 7) in HeLa and NIH/3T3 cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H2A.Z (Lys 7) in Histone peptides with various modifications.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl Histone H2A.Z (Lys 7) in 500 µg of HeLa acid extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected monomethyl Histone H2A.Z (Lys 7) in 10 µg of HeLa acid extract.

Target description

~16 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable