EZBRAIN42

Human Amyloid β42 ELISA Kit

measures and quantifies Amyloid β42 levels in 50 μL tissue or cell lysates

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.77

Properties

• Product Name: Human Amyloid β42 Brain ELISA, This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
• Quality Level: 200
• species reactivity: human
• packaging: kit of 1 × 96 wells
• parameter: 50 μL sample volume (Overnight assay)
• assay range: sensitivity: 8 pg/mL; (lowest level of Amyloid β1-42 standard; 50 μL sample size); standard curve range: 16-500 pg/mL
• technique(s): ELISA: suitable
• input: sample type tissue/cellular lysate
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... APP(351)

Description

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

Specificity

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Application

Research Category
Neuroscience

Research Sub Category
Alzheimer′s Disease

This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.

This assay requires 50 µl of sample and is an overnight assay.

Used to detect/quantify: Amyloid β42

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Warning
• Hazard Statements: H290,H317,H412
• Precautionary Statements: P234 - P261 - P272 - P273 - P280 - P302 + P352
• Hazard Classifications: Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials