PC223L
Anti-Neuropeptide Y Rabbit pAb
lyophilized, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
human, avian, amphibian, fish, feline, rat, newt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
General description
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.
Recognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.
This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.
Immunogen
porcine neuropeptide Y
Application

Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)
Warning
Toxicity: Standard Handling (A)
Reconstitution
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Paraventricular nucleus, cortex, or caudate putamen
Paraventricular nucleus, cortex, or caudate putamen
Other Notes
Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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