90903

Schaeffer and Fulton Spore Stain Solution A

• Synonym(s): Malachite Green Solution according to Schaeffer and Fulton
• CAS Number: 2437-29-8
• MDL number: MFCD06412418
• UNSPSC Code: 12171500
• NACRES: MA.02

Properties

• product line: BioChemika
• form: solution
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• suitability: suitable for microscopy; spore forming microorganisms
• SMILES string: [N+](=C4C=CC(=C(c6ccc(cc6)N(C)C)c5ccccc5)C=C4)(C)C.[N+](=C1C=CC(=C(c3ccc(cc3)N(C)C)c2ccccc2)C=C1)(C)C.[O-]C(=O)C(=O)[O-].OC(=O)C(=O)O.OC(=O)C(=O)O
• InChI: 1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2
• InChI key: CNYGFPPAGUCRIC-UHFFFAOYSA-L

Description

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin (Schaeffer & Fulton`s Spore Stain B, Cat. No. 39955). The components and the method are also available as kit (Cat. No. 04551).

Components

Composition:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water

Safety Information

• Pictograms: GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H318,H361fd,H411
• Precautionary Statements: P202 - P273 - P280 - P305 + P351 + P338 - P308 + P313 - P391
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves