909475

5-Ethynyl uridine

≥95%

• Synonym(s): 5-EU, EU
• Empirical Formula (Hill Notation): C₁₁H₁₂N₂O₆
• CAS Number: 69075-42-9
• Molecular Weight: 268.22
• MDL number: MFCD00876719
• UNSPSC Code: 41106305
• NACRES: NA.22

Properties

• Assay: ≥95%
• form: powder
• reaction suitability: reaction type: click chemistry
• storage temp.: −20°C
• SMILES string: [nH]1[c]([n](cc([c]1=O)C#C)[C@@H]2O[C@@H]([C@H]([C@H]2O)O)CO)=O
• InChI: 1S/C11H12N2O6/c1-2-5-3-13(11(18)12-9(5)17)10-8(16)7(15)6(4-14)19-10/h1,3,6-8,10,14-16H,4H2,(H,12,17,18)/t6-,7-,8-,10-/m1/s1
• InChI key: QCWBIPKYTBFWHH-FDDDBJFASA-N

Description

Application

This alkyne-bearing metabolic labeling reagent can be used to measure RNA synthesis in proliferating cells. Once fed to cells, this nucleoside is incorporated during active RNA synthesis and can then be detected using a copper-catalyzed click reaction with fluorescent dyes. The cells can then be analysed by fluorescence imaging, flow cytometry or high content imaging analysis.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable