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Key Documents

909475

Sigma-Aldrich

5-Ethynyl uridine

≥95%

Synonym(s):

5-EU, EU

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About This Item

Empirical Formula (Hill Notation):
C11H12N2O6
CAS Number:
Molecular Weight:
268.22
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.22

Assay

≥95%

form

powder

reaction suitability

reaction type: click chemistry

storage temp.

−20°C

SMILES string

[nH]1[c]([n](cc([c]1=O)C#C)[C@@H]2O[C@@H]([C@H]([C@H]2O)O)CO)=O

InChI

1S/C11H12N2O6/c1-2-5-3-13(11(18)12-9(5)17)10-8(16)7(15)6(4-14)19-10/h1,3,6-8,10,14-16H,4H2,(H,12,17,18)/t6-,7-,8-,10-/m1/s1

InChI key

QCWBIPKYTBFWHH-FDDDBJFASA-N

Application

This alkyne-bearing metabolic labeling reagent can be used to measure RNA synthesis in proliferating cells. Once fed to cells, this nucleoside is incorporated during active RNA synthesis and can then be detected using a copper-catalyzed click reaction with fluorescent dyes. The cells can then be analysed by fluorescence imaging, flow cytometry or high content imaging analysis.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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