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Key Documents

E4009

Sigma-Aldrich

Eosin Y

Dye content ~99%, Powder

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43

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About This Item

Empirical Formula (Hill Notation):
C20H8Br4O5
CAS Number:
Molecular Weight:
647.89
Colour Index Number:
45380:2
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Product Name

Eosin Y, Dye content ~99 %

form

powder

Quality Level

composition

Dye content, ~99%

color

light red to red/pink

solubility

ethanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

DBZJJPROPLPMSN-UHFFFAOYSA-N

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Application

Eosin Y has been used as a stain:
  • to determine the location of the brain sections
  • for staining of fixed liver tissue
  • for histological examination of brain sections from the dorsal hippocampus

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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