E7889

Ethylenediaminetetraacetic acid disodium salt solution

0.5 M in H₂O, DNase, RNase, NICKase and protease, none detected

• Synonym(s): (Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na₂
• Linear Formula: [-CH₂N(CH₂CO₂Na)CH₂CO₂H]₂
• CAS Number: 139-33-3
• Molecular Weight: 336.21
• Beilstein: 3822669
• MDL number: MFCD00070672
• UNSPSC Code: 12352107
• PubChem Substance ID: 24894660
• NACRES: NA.31

Properties

• grade: Molecular Biology
• Quality Level: 300
• sterility: 0.2 μm filtered
• form: liquid
• reaction suitability: reagent type: chelator
• concentration: 0.5 M in H₂O
• pH: 7.5-8.5
• suitability: suitable for molecular biology
• application(s): microbiology
• foreign activity: DNase, RNase, NICKase and protease, none detected
• SMILES string: [Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2
• InChI key: ZGTMUACCHSMWAC-UHFFFAOYSA-L

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.

Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:

• in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
• with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
• in pretreatment for detaching epithelial cells grown to 80% confluency
• to prepare stock solutions for lysis buffer
• in the preparation of urea lysis buffer

Features and Benefits

molecular biology grade

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H373
• Precautionary Statements: P260 - P314 - P501
• Hazard Classifications: STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable