Y1500

YPD Agar

• Synonym(s): Yeast Extract Peptone Dextrose Agar, YEPD Agar
• UNSPSC Code: 50405901
• eCl@ss: 42040102
• NACRES: NA.75

Properties

• form: powder
• Quality Level: 200
• composition: Agar, 15 g/L ; Bacteriological peptone, 20 g/L ; Glucose, 20 g/L ; Yeast extract, 10 g/L
• application(s): food and beverages
• suitability: nonselective for Candida spp.; nonselective for Pichia spp.; nonselective for Saccharomyces spp.; nonselective for Zygosaccharomyces spp.

Description

General description

YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.

Application

YPD agar has been used as a culture media:

• to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
• for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
• for Zymoseptori triciti strains for virulence studies

Preparation Note

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves