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biological source
human lymph
form
liquid
growth mode
Non-adherent or adherent on poly-L-lysine treated plastic
karyotype
2n=46
morphology
Lymphoblast
products
Adult T-cell leukaemia virus (ALT; HTLV-1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
-65 to -96°C
Cell Line Origin
Human T cell leukaemia
Cell Line Description
Derived from a 45 year old Japanese female with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of her peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. MT-2 has a male karyotype and the presence of a Y chromosome has been confirmed by STR profiling at ECACC
DNA Profile
Not specified
Culture Medium
RPMI 1640 + 2 mM Glutamine + 10% Decomplemented Fetal Bovine Serum (FBS).
Subculture Routine
Seed cultures between 2-3x100,000 cells/ml. Saturation density 9 x 100,000 cells/ml; 5% CO2; 37 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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