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46393

Sigma-Aldrich

Fluo-3

suitable for fluorescence, ~70%

Synonym(s):

4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid

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3 EA
CA$738.00

CA$738.00

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3 EA
CA$738.00

About This Item

Empirical Formula (Hill Notation):
C36H30Cl2N2O13
CAS Number:
123632-39-3
Molecular Weight:
769.54
MDL number:
MFCD00083337
UNSPSC Code:
12352106
PubChem Substance ID:
329757162
NACRES:
NA.32

CA$738.00

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Assay

~70%

Quality Level

100

form

solid

solubility

H2O: soluble

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1

InChI

1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

InChI key

OZLGRUXZXMRXGP-UHFFFAOYSA-N

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This Item
KWLSA04HH3KWL9A04HH3KWL6A04HH3
Milligard® LPB 1.2/0.5 µm nominal, Opticap® XL Capsule Opticap® XL 4, inlet connection diam. 9/16 in., pore size 1.2/0.5 μm, cartridge nominal length 4 in. (10 cm)

KWLCA04HH3

Milligard® LPB 1.2/0.5 µm nominal, Opticap® XL Capsule

Milligard® LPB 0.5/0.2 µm nominal, Opticap® XL Capsule Opticap® XL 3, inlet connection diam. 9/16 in., pore size 0.5/0.2 μm, cartridge nominal length 4 in. (10 cm)

KWLSA04HH3

Milligard® LPB 0.5/0.2 µm nominal, Opticap® XL Capsule

Milligard® LPB 1.2 µm, Opticap® XL Capsule inlet connection diam. 9/16 in., Opticap® XL 4, pore size 1.2 μm, cartridge nominal length 4 in. (10 cm)

KWL9A04HH3

Milligard® LPB 1.2 µm, Opticap® XL Capsule

Milligard® LPB 0.2 µm nominal, Opticap® XL Capsule inlet connection diam. 9/16 in., Opticap® XL 4, pore size 0.5 μm, cartridge nominal length 4 in. (10 cm)

KWL6A04HH3

Milligard® LPB 0.2 µm nominal, Opticap® XL Capsule

pore size

1.2/0.5 μm nominal pore size, 1.2/0.5 μm pore size

pore size

0.5/0.2 μm nominal pore size, 0.5/0.2 μm pore size

pore size

-

pore size

-

product line

Opticap® XL 4

product line

Opticap® XL 3

product line

Opticap® XL 4

product line

Opticap® XL 4

filtration area

0.16 m2

filtration area

0.16 m2

filtration area

0.2 m2

filtration area

0.2 m2

technique(s)

prefiltration: suitable

technique(s)

prefiltration: suitable

technique(s)

prefiltration: suitable

technique(s)

prefiltration: suitable

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

matrix

Milligard® LPB

matrix

Milligard® LPB

matrix

Milligard® LPB

matrix

Milligard® LPB

Application

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding.

Other Notes

Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding[1][2]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCF4708
    BCCC6119
    BCBW6132
    BCBS0366V
    BCBN0298V

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