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Key Documents

51411C

SAFC

Minimum Essential Medium

with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Minimum Essential Medium Eagle, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro . MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle′s Balanced Salts, which make them suitable for use in atmospheres charged with CO2.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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