93711

Atto 655

BioReagent, suitable for fluorescence, ≥85% (HPLC)

• MDL number: MFCD03456136
• UNSPSC Code: 12352116
• PubChem Substance ID: 57653573
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥85% (HPLC)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm; 655 nm
• fluorescence: λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 652-658 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: [S](=O)(=O)([O-])CC1CC(N(c2c1cc3c(c2)OC4=CC5=[N+](CCCC5=CC4=N3)CC)CCCC(=O)O)(C)C
• InChI: 1S/C27H33N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-14,18H,4-10,15-16H2,1-3H3,(H-,31,32,33,34,35)
• InChI key: FOYVTVSSAMSORJ-UHFFFAOYSA-N

Description

Application

Atto labels are designed for highest sensitivity applications. A unique combination of advantages makes them highly favorable tools for all kinds of labeling applications. Some of their properties make them specifically interesting for single molecule detection. Atto labels are based on rigid structures and do not show any cis-trans-isomerization, which lowers the brightness of signals and leads to environment dependency, e.g., spectral shifts by conjugation.
Atto 655 shows a molar extinction of 110,000 and QY of 30% in water (50% in ethanol). Decay time is 1.9 ns.

Other Notes

New red absorbing fluorescent dye with best signal-to-noise ratio and long fluorescence life-time. Useful as a biophysical probe for binding interactions.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves