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C0150

Sigma-Aldrich

Cathepsin B from human placenta

lyophilized powder, ≥5 units/mg protein

Synonym(s):

Cathepsin B1

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About This Item

CAS Number:
9047-22-7
EC Number:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD00130748
UNSPSC Code:
12352204
NACRES:
NA.54
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form

lyophilized powder

Quality Level

200

specific activity

≥5 units/mg protein

mol wt

24.5 kDa

composition

Protein, ~50% Lowry

solubility

H2O: soluble 1.0 mg/mL, clear

UniProt accession no.

P07858

storage temp.

−20°C

Gene Information

human ... CTSB(1508)

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4-methoxyindole-3-carboxylic acid AldrichCPR

CDS006454

4-methoxyindole-3-carboxylic acid

4-methoxyindole-3-carboxaldehyde AldrichCPR

CDS004557

4-methoxyindole-3-carboxaldehyde

5,6-Dimethoxyindole-2-carboxylic acid AldrichCPR

CDS000930

5,6-Dimethoxyindole-2-carboxylic acid

5-Methoxyindole-2-carboxylic acid 97%

M14951

5-Methoxyindole-2-carboxylic acid

form

solid

form

solid

form

solid

form

-

General description

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1.[1] Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.[2]

Application

Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG).[3] It has also been used in determining the effect of cysteine protease inhibitors.[2]
Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease [4]. Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

Biochem/physiol Actions

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments [4]. Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer [5].

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Lyophilized powder containing phosphate buffer salts

Preparation Note

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    014K37832
    104K3781
    116H3838
    081H38162
    074H3864

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