A1983

AURORA A, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• product line: PRECISIO^® Kinase
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 62-84 nmol/min·mg
• mol wt: ~72 kDa
• UniProt accession no.: O14965
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... AURKA(6790)

Description

Biochem/physiol Actions

AURORA A belongs to a multigenic family of mitotic serine/threonine kinases which are involved in the control of chromosome segregation. AURORA A is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. AURORA A is expressed and active at the highest level during G2-M phase of the cell cycle. Overexpression of AURORA A has been found to be correlated with the grade of various human solid tumours. Ectopic AURORA A overexpression in any culture cell line leads to polyploidy and centrosome amplification.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves