A2714

Aldolase from rabbit muscle

lyophilized powder, ≥8.0 units/mg protein

• Synonym(s): D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
• CAS Number: 9024-52-6
• EC Number: 4.1.2.13 (BRENDA, IUBMB)
• EC Number: 232-781-0
• MDL number: MFCD00130453
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 300
• form: lyophilized powder
• specific activity: ≥8.0 units/mg protein
• composition: Protein, ≥80% biuret
• foreign activity: glyceraldehyde-3-phosphate dehydrogenase ≤0.03%; lactic dehydrogenase ≤0.03%; phosphoglucose isomerase ≤0.6%; pyruvate kinase ≤0.1%; triosephosphate isomerase ≤0.05%
• storage temp.: −20°C

Description

Application

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts.

Biochem/physiol Actions

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold .

Unit Definition

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Physical form

Essentially sulfate-free containing citrate buffer salts

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves