A5384

Aconitase from porcine heart

• Synonym(s): Aconitate hydratase, Citrate (isocitrate) hydrolyase
• CAS Number: 9024-25-3
• EC Number: 4.2.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00130335
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Porcine heart
• Quality Level: 200
• form: solid
• mol wt: ~66 kDa by gel filtration
• storage temp.: −20°C

Description

Application

Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .

Biochem/physiol Actions

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

Unit Definition

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

Analysis Note

Crude preparation containing aconitase activity

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves