A7080

Amphiregulin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): AREG, CRDGF, SDGF, AR
• CAS Number: 117147-70-3
• MDL number: MFCD00282118
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Product Name: Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 10-100 ng/mL ED₅₀/EC₅₀
• mol wt: ~11 kDa
• packaging: pkg of 1 mg
• storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P15514
• storage temp.: −20°C
• Gene Information: human ... AREG(374)

Description

Biochem/physiol Actions

Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.

Components

Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.

Preparation Note

It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.

Analysis Note

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED₅₀ for this effect is typically 5-15 ng/mL.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves