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Key Documents

A9186

Sigma-Aldrich

Alcian Blue 8GX

BioReagent, suitable for detection of glycoproteins

Synonym(s):

Alcian Blue, Ingrain Blue 1

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About This Item

Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
Molecular Weight:
1298.86
Colour Index Number:
74240
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

form

powder

composition

Dye content, ~50%

technique(s)

electrophoresis: suitable

suitability

suitable for detection of glycoproteins

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

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General description

Alcian Blue 8GX is a cationic phthalocyanine dye. It has an ability to interact with sulphated glycosaminoglycan. It is used as a counterstain to visualize histological tissue sections. In addition, alcian blue 8GX also aids in the in-situ quantification of marine biofilms produced by marine bacteria.

Application

Alcian Blue 8GX has been used:
  • for the quantification of sulphated glycosaminoglycan
  • to stain differentiated chondrocytes to access proteoglycan′s synthesis by these cells
  • to stain Multi-lineage differentiation of hemangioma-derived perivascular cells (Hem-PCs)
  • in histological staining to detect neutral and charged carbohydrate residues

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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