A9231

Acridine Orange solution

2% in H₂O

• Synonym(s): 3,6-Acridinediamine, N,N,N′,N′-tetramethyl-, monohydrochloride, 3,6-Bis(dimethylamino)acridine hydrochloride
• Empirical Formula (Hill Notation): C₁₇H₁₉N₃ · HCl
• CAS Number: 65-61-2
• Molecular Weight: 301.81
• MDL number: MFCD00005029
• UNSPSC Code: 12171500
• PubChem Substance ID: 329771043
• NACRES: NA.47

Properties

• form: liquid
• Quality Level: 100
• shelf life: 18 mo.
• concentration: 2% in H₂O
• technique(s): microbe id | staining: suitable
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: N(C)(C)c1cc2nc3c(cc2cc1)ccc(c3)N(C)C
• InChI: 1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
• InChI key: DPKHZNPWBDQZCN-UHFFFAOYSA-N

Description

General description

Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.

Application

Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.

It also has the following applications-

• staining of RNA and acid mucins
• detecting apoptosis
• staining blood smears to diagnose malaria
• identifying and enumerating granulocytes in fish
• staining eukaryotic cells to demonstrate low pH structures

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves