C2888

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

• Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
• CAS Number: 9000-81-1
• EC Number: 3.1.1.7 (BRENDA, IUBMB)
• EC Number: 232-559-3
• MDL number: MFCD00081268
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: Electrophorus electricus
• Quality Level: 200
• type: Type V-S
• form: lyophilized powder
• specific activity: ≥1,000 units/mg protein
• mol wt: 280 kDa
• composition: Protein, ≥45% biuret
• pI: ~5.3
• solubility: Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
• storage temp.: −20°C

Description

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E^1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:

• in acetylcholinesterase activity assay
• to study its effects on Xenopus embryonic hippocampal cells
• to study the effect of acetylcholine inhibitors on development and plasticity

Biochem/physiol Actions

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Caution

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves