C4963

Catalase−polyethylene glycol

lyophilized powder, ~40,000 units/mg protein

• Synonym(s): PEG-Catalase
• MDL number: MFCD00676653
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ~40,000 units/mg protein
• mol wt: PEG 5,000
• composition: Protein, ~50% E₄₀₅
• extent of labeling: ~40 mol PEG per mol protein
• matrix attachment: secondary amine linkage.
• storage temp.: −20°C

Description

Application

Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.

Biochem/physiol Actions

Catalase from bovine liver catalyzes the decomposition of H₂O₂ into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu²⁺ results in degradation of the catalase molecule. It does not require any activators.

Packaging

Package size based on protein content

Other Notes

Catalase from bovine liver coupled to methoxy-polyethylene glycol.

Physical form

Contains PEG plus 5% citrate buffer salts

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves