C5338

Cyanogen bromide-activated-Sepharose^™ 4 Fast Flow

lyophilized powder

• Synonym(s): Cyanogen bromide-activated Agarose
• CAS Number: 68987-32-6
• MDL number: MFCD00163385
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• form: lyophilized powder
• Quality Level: 200
• technique(s): affinity chromatography: suitable
• matrix: Sepharose 4 Fast Flow
• matrix active group: cyanate or related structures
• matrix spacer: 1 atom (when ligands are coupled via isourea derivative or related linkage)
• swelling: 1 g swells to 4-5 mL gel
• particle size: 45—165 μm
• capacity: 6-11 μmol/mL coupling capacity (6-AKS)
• storage temp.: 2-8°C

Description

General description

C5338-10G′s update product number is GE17-0981-01

Cyanogen bromide-activated Sepharose^™ 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

Application

Cyanogen bromide-activated-Sepharose^™ is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose^™ has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Physical form

Lyophilized powder with sugar stabilizers

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves