RPMI 2650

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88031602, human nose, Epithelial

• Synonym(s): RPMI Cell Culture Medium
• UNSPSC Code: 41106514

Properties

• biological source: human nose
• packaging: tube of 5 μg 88031602-DNA-5UG; pkg of vial of cells 88031602-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Mucoid material
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human nasal septum quasidiploid tumour

Cell Line Description

From the pleural effusion of an extensive malignant tumour of the nasal septum, diagnosed as anaplastic squamous cell carcinoma. Nearly normal karyotype, stability of chromosome number and probable origin from malignant cells make the cell line unique amongst permanent human cell lines. Cells are very small and grow in clusters fusing to form thick layers.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.