CMC0019

OverExpress^™ C43(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

• Synonym(s): C43 strain
• UNSPSC Code: 41106202
• NACRES: NA.85

Properties

• Product Name: OverExpress^™ C43(DE3) Chemically Competent Cells, for the highest protein expression
• biological source: Escherichia coli
• grade: Molecular Biology
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: chemically competent; transformation efficiency: ≥1 x 10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

OverExpress^™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress^™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress^™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

OverExpress^™ C43(DE3) Chemically Competent Cells have been used for transforming the toll/interleukin receptor (TIR) domain constructs of sterile alpha and TIR motif–containing protein 1 (SARM1).

Features and Benefits

• Express genes cloned into any T7 vector with these BL21(DE3) derivatives
• Effective in expressing toxic & membrane proteins
• Cited in over 350 research articles

Components

• OverExpress C43(DE3) chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Legal Information

OverExpress is a trademark of Imaxio, S.A.

Safety Information

• Storage Class Code: 10 - Combustible liquids