CS0170

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

• Synonym(s): Thioredoxin Reductase Activity Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 200
• usage: kit sufficient for 100 assays (1 mL)
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

Description

General description

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H⁺ ↔ 2 TNB + NADP⁺

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Features and Benefits

• An easy and simple method
• The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
• The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
• The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Kit Components Only

• Assay Buffer 5x for Thioredoxin Reductase 30 mL
• Thioredoxin Reductase 10 μg
• Thioredoxin Reductase Inhibitor Solution .05 mL
• 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
• NADPH 25 mg
• Dimethyl Sulfoxide (DMSO) 7.5 mL

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H361
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C