E8909

Phosphodiesterase 3B

recombinant, expressed in Sf9 cells

• Synonym(s): 3′,5′-cyclic-nucleotide phosphodiesterase, PDE 3B
• EC Number: 3.1.4.17 (BRENDA, IUBMB)
• MDL number: MFCD00131864
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in Sf9 cells
• Quality Level: 200
• form: buffered aqueous solution
• specific activity: ≥1000 units/μg protein
• shipped in: dry ice
• storage temp.: −70°C

Description

Application

Phosphodiesterase 3B has been used in a study to evaluate the evidence for biological effects of metformin in operable breast cancer. Phosphodiesterase 3B has also been used in a study to investigate the role of intracellular and intercellular communication in perfusion distribution of erythrocyte-derived ATP.

Physical properties

N-terminal GST-tagged 86 kDa protein containing amino acids 592-end

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cAMP to 5′-AMP per minute at pH 7.4 at 37 °C.

Physical form

Buffered aqueous solution containing Tris-HCl (pH 8.0), NaCl, KCl, Tween-20, DTT, and glycerol

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable