G25150

Sephadex^® G-25

Medium

• Synonym(s): Sephadex G-25 Medium, Sephadex^® G-25 resins, Size exclusion resins
• CAS Number: 9041-35-4
• MDL number: MFCD00132770
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• Quality Level: 200
• technique(s): buffer exchange: suitable
• matrix active group: phase
• swelling: 1 g swells to 4-6 mL
• bead size: 50-150 μm
• application(s): life science and biopharma
• compatibility: Cytiva
• InChI: 1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
• InChI key: BCPVZFFJGCURNR-OCOFDJSDSA-N

Description

General description

Sephadex^® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.

Application

Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000

Sephadex^® G-25 has been used:

• in the desalting of protease
• in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation

Sephadex^® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.

Biochem/physiol Actions

Sephadex^® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex^® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.

Features and Benefits

• Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
• High recovery rate with minimal sample dilution.
• Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
• Specifically designed as a BioProcess resin for industrial applications.

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves