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G25150

Sigma-Aldrich

Sephadex® G-25

Medium

Synonym(s):

Sephadex G-25 Medium, Sephadex® G-25 resins, Size exclusion resins

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50 G
CA$106.00

CA$106.00


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50 G
CA$106.00

About This Item

CAS Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

CA$106.00


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Quality Level

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 4-6 mL

bead size

50-150 μm

application(s)

life science and biopharma

compatibility

Cytiva

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

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1 of 4

This Item
1.0090101202141.46142
suitability

Cronobacter spp., enterobacteriaceae, Salmonella spp.

suitability

Cronobacter spp., Salmonella spp., enterobacteriaceae

suitability

Citrobacter ssp., Cronobacter ssp., Escherichia coli, Klebsiella ssp., Proteus ssp., Providencia ssp., Pseudomonas ssp., Salmonella spp., Shigella ssp., Staphylococcus ssp., Streptococcus ssp., Yersinia ssp., bacteria, coliforms, enterobacteriaceae

suitability

Salmonella spp.

agency

APHA, ISO 21528, ISO 6579, EP, ISO 22964

agency

APHA, EP, FDA-BAM, ISO 21528, ISO 22964, according to ISO 6579

agency

GB, ISO 6579, ISO 6887, according to GB 4789.30-2016, according to GB 4789.40-2016, according to ISO 21528-1:2017, according to ISO 22964:2017

agency

EP, according to ISO 6887

manufacturer/tradename

ReadyBag®

manufacturer/tradename

ReadyBag®

manufacturer/tradename

-

manufacturer/tradename

ReadyTube®

form

granular

form

granular

form

liquid

form

liquid (ready-to-use)

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
industrial qc
microbiology
pathogen testing
pharmaceutical

application(s)

environmental
food and beverages
industrial qc
microbiology
pathogen testing
sample preparation

application(s)

cosmetics
environmental
food and beverages
microbiology

General description

Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.

Application

Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000

Sephadex® G-25 has been used:
  • in the desalting of protease[1]
  • in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation[2]
Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.

Biochem/physiol Actions

Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.

Features and Benefits

  • Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
  • High recovery rate with minimal sample dilution.
  • Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
  • Specifically designed as a BioProcess resin for industrial applications.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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