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G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

Synonym(s):

Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

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100 μL
₩8,19,613

About This Item

CAS Number:
9031-68-9
EC Number:
2.4.1.22 (BRENDA, IUBMB)
MDL number:
MFCD00131122
UNSPSC Code:
12352204
NACRES:
NA.54

₩8,19,613

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form

lyophilized powder

Quality Level

200

specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

composition

Protein, 35-65% modified Warburg-Christian

impurities

≤10 % protein α-lactalbumin

storage temp.

−20°C

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1 of 4

This Item
SAE0093L2005L0130
Galactosyltransferase from bovine milk lyophilized powder

G5507

Galactosyltransferase from bovine milk

Beta-1,4-galactosyltransferase 1 B4GALT1 human recombinant, expressed in HEK 293 cells, 2000 units/mg protein

SAE0093

Beta-1,4-galactosyltransferase 1

Lactoperoxidase from bovine milk lyophilized powder (essentially salt-free), ≥200 units/mg protein

L2005

Lactoperoxidase from bovine milk

β-Lactoglobulin from bovine milk ≥90% (PAGE), lyophilized powder

L0130

β-Lactoglobulin from bovine milk

specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

specific activity

2000 units/mg protein

specific activity

≥200 units/mg protein

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder (essentially salt-free)

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

impurities

≤10 % protein α-lactalbumin

impurities

-

impurities

-

impurities

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

300

composition

Protein, 35-65% modified Warburg-Christian

composition

-

composition

-

composition

-

General description

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.[1]Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.[2]

Application

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1[3]
  • in β -1,4-galactosyltransferase assay rice root cell extracts[4]
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers[5]

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biochem/physiol Actions

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides[6].
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality.[7] Alteration in the galactosyltransferase levels have been implicated in various disease states.[2]Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.[1]

Linkage

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Unit Definition

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Physical form

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Lot/Batch Number
    SLCJ0342
    SLBC8723V
    SLBB3943V
    TESTNAM003
    SLBB0299V

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    Anika Steffen et al.
    The EMBO journal, 23(4), 749-759 (2004-02-07)
    The Rho-GTPase Rac1 stimulates actin remodelling at the cell periphery by relaying signals to Scar/WAVE proteins leading to activation of Arp2/3-mediated actin polymerization. Scar/WAVE proteins do not interact with Rac1 directly, but instead assemble into multiprotein complexes, which was shown
    Local F-actin network links synapse formation and axon branching.
    Chia, PH; Chen, B; Li, P; Rosen, MK; Shen, K
    Cell null
    Patricia Kunda et al.
    Current biology : CB, 13(21), 1867-1875 (2003-11-01)
    In animal cells, GTPase signaling pathways are thought to generate cellular protrusions by modulating the activity of downstream actin-regulatory proteins. Although the molecular events linking activation of a GTPase to the formation of an actin-based process with a characteristic morphology
    Marco A Salazar et al.
    The Journal of biological chemistry, 278(49), 49031-49043 (2003-09-25)
    Tuba is a novel scaffold protein that functions to bring together dynamin with actin regulatory proteins. It is concentrated at synapses in brain and binds dynamin selectively through four N-terminal Src homology-3 (SH3) domains. Tuba binds a variety of actin
    Reply to "Reduced CYFIP2 Stability by Arg87 Variants Causing Human Neurological Disorders".
    Mitsuko Nakashima et al.
    Annals of neurology, 86(5), 805-806 (2019-09-12)
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