G7509

GABase from Pseudomonas fluorescens

lyophilized powder, Protein ≥30 % by biuret

• Synonym(s): GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase
• MDL number: MFCD00131113
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: Pseudomonas fluorescens
• Quality Level: 200
• Assay: ≥30 % (biuret)
• form: lyophilized powder
• specific activity: ≥0.5 units/mg protein
• composition: Protein, ≥30% biuret
• technique(s): inhibition assay: suitable
• storage temp.: −20°C

Description

General description

Research area: Neuroscience


GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.

Application

GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).

Biochem/physiol Actions

GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.

Unit Definition

One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP⁺ at pH 8.6 at 25 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves