H1506

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

• Synonym(s): 3α-Hydroxysteroid:NAD(P)⁺ oxidoreductase
• CAS Number: 9028-56-2
• EC Number: 1.1.1.50 (BRENDA, IUBMB)
• EC Number: 232-839-5
• MDL number: MFCD00130298
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Pseudomonas testosteroni)
• Quality Level: 200
• form: lyophilized solid
• specific activity: ≥15 units/mg protein
• purified by: chromatography
• composition: Protein, ≥25%
• foreign activity: β-hydroxysteroid dehydrogenase ≤0.5%; alcohol dehydrogenase ≤0.05%
• storage temp.: −20°C

Description

Application

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:

• in the preparation of composite electrode platform to for the determination of androsterone
• in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
• for total bile salts assay using an enzymatic method

Biochem/physiol Actions

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Unit Definition

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD⁺.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves