H3757

Hyaluronidase from bovine testes

Type VIII, lyophilized powder, 300-1,000 U/mg

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• EC Number: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine testis
• Quality Level: 200
• conjugate: conjugate (Glucosaminoglycan)
• type: Type VIII
• form: lyophilized powder
• specific activity: 300-1,000 U/mg
• mol wt: ~55 kDa (four subunits of 14 kDa each)
• UniProt accession no.: Q5E985; Q8SQG8
• relevant disease(s): cancer
• storage temp.: −20°C
• Gene Information: cow ... HYAL1(515397), HYAL2(281838)

Description

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Preparation Note

Prepared from aseptic filled solution of Type I-S.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves